detection_of_recombination_in_bacterial_genomes
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detection_of_recombination_in_bacterial_genomes [2018/06/18 08:58] – [Gubbins] hyjeong | detection_of_recombination_in_bacterial_genomes [2021/03/17 13:09] (current) – external edit 127.0.0.1 | ||
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- | $ snippy --cpus 16 outdir mysnps --ref E_coli.gbk --R1 read_1.fastq.gz --R2 read_2.fastq.gz | + | $ snippy --cpus 16 --outdir mysnps --ref E_coli.gbk --R1 read_1.fastq.gz --R2 read_2.fastq.gz |
$ snippy-core --prefix core mysnps1 mysnps2 mysnps2 | $ snippy-core --prefix core mysnps1 mysnps2 mysnps2 | ||
snippy-core 결과물인 core.full.aln(whole genome SNP alignment including invariant sites)을 gubbins에 이용하면 된다. | snippy-core 결과물인 core.full.aln(whole genome SNP alignment including invariant sites)을 gubbins에 이용하면 된다. | ||
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$ run_gubbins.py dataset.aln | $ run_gubbins.py dataset.aln | ||
+ | $ gubbins_drawer.py -o result.pdf dataset.final_tree.tre dataset.recombination_predictions.embl | ||
+ | $ gubbins_drawer.py -o results_SNPS.pdf dataset.final_tree.tre dataset.branch_base_reconstruction.embl | ||
===== Visualization using phandango ===== | ===== Visualization using phandango ===== | ||
detection_of_recombination_in_bacterial_genomes.1529279926.txt.gz · Last modified: (external edit)