bioinfo:long_read_sequencing_결과물_다루기
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bioinfo:long_read_sequencing_결과물_다루기 [2023/08/17 09:32] – [UniCycler] hyjeong | bioinfo:long_read_sequencing_결과물_다루기 [2023/08/17 09:33] (current) – [Long read의 reference mapping] hyjeong | ||
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==== Assembly graph의 구조 확인 ==== | ==== Assembly graph의 구조 확인 ==== | ||
+ | LastGraph(Velvet), | ||
+ | |||
+ | $ Bandage load assembly_graph.gfa | ||
+ | |||
+ | 인수 없이 Bandage라고만 입력하면 Bandage GUI가 작동하게 되며, File -> Load graph에서 그래프를 로드한 뒤 ‘Drawu graph’ 버튼을 클릭하면 화면에 시각화된 그래프가 표현된다. | ||
===== Long read의 reference mapping ===== | ===== Long read의 reference mapping ===== | ||
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$ minimap2 -ax map-ont ref.fa ont-reads.fq > aln.sam # for Oxford Nanopore reads | $ minimap2 -ax map-ont ref.fa ont-reads.fq > aln.sam # for Oxford Nanopore reads | ||
# Convert SAM to BAM | # Convert SAM to BAM | ||
- | $ samtools view –b –S –o aln.bam aln.sam # samtools version | + | $ samtools view –b –S –o aln.bam aln.sam # samtools version |
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bioinfo/long_read_sequencing_결과물_다루기.1692232321.txt.gz · Last modified: by hyjeong