Differences

This shows you the differences between two versions of the page.

--- application_of_pacbio_long_reads_sequencing_technology [2018/11/30 09:08] – [phh5tools] hyjeong
+++ application_of_pacbio_long_reads_sequencing_technology [2021/03/17 13:09] (current) – external edit 127.0.0.1
@@ Line 8: / Line 8: @@
   * [[https://github.com/PacificBiosciences/Bioinformatics-Training/wiki|PacBio bioinformatics training wiki]]
-===== phh5tools =====
+===== pbh5tools =====
 A Swiss-army knife for interrogating PacBio HDF5 files (cmp.h5, bas.h5)
   * https://github.com/PacificBiosciences/pbh5tools
@@ Line 29: / Line 29: @@
   - (--readType subreads --minReadScore 0.8): 1287559618 bp / 118740 seqs; 10843.5 average length
   - (--readType subreads --minReadScore 0.85): 1038658565 bp / 92346 seqs; 11247.5 average length
-  - (--readType subreads --minReadScore 0.9):
+  - (--readType subreads --minReadScore 0.9): 654807 bp / 121 seqs; 5411.6 average length
-  - (--readType subreads --minReadScore 0.95):
+  - (--readType subreads --minReadScore 0.95): no sequence extracted!
+--minReadScore가 0.9에 근접하면서 결과물의 분량이 현저히 떨어진다. --minReadScore 0.75로 하는 것이 Analysis Results 서브디렉토리에 있는 subreads file의 분량과 거의 흡사하다.
 ===== SMRT analysis =====
   * SMRT analysis system requirements [[http://www.pacb.com/wp-content/uploads/2015/09/SMRT-Analysis-System-Requirements.pdf|PDF 문서]]