====== Primer design ======

[[:sars-cov-2_검출을_위한_pcr_프라이머_설계|SARS-CoV-2 검출을 위한 PCR 프라이머 설계]]

MSA-based... 관련 도구 모음

  * [[http://trimal.cgenomics.org/|trimAl]] https://github.com/inab/trimal
  * [[https://github.com/JLSteenwyk/ClipKIT|ClipKIT]]
  * [[https://github.com/desmid/mview|MView]]
  * [[https://sourceforge.net/projects/andestools/|ANDES: Statistical tools for the ANalyses of DEep Sequencing]]
  * [[https://www.hiv.lanl.gov/content/sequence/PRIMER_DESIGN/primer_design.html|PrimerDesign-M]]
  * [[https://github.com/MatteoPS/EasyPrimer|EasyPrimer]]

설계된 프라이머의 evaluation

  * [[https://www.thermofisher.com/kr/ko/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/multiple-primer-analyzer.html|Multiple Primer Analyzer]]
  * https://mfeprimer3.igenetech.com/spec